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| Targon,Maria Luísa P. Natividade; Takita,Marco Aurélio; Amaral,Alexandre M. do; Souza,Alessandra A. de; Locali-Fabris,Eliane Cristina; Dorta,Sílvia de Oliveira; Borges,Kleber Martins; Souza,Juliana Mendonça de; Rodrigues,Carolina Munari; Lucheta,Adriano Reis; Freitas-Astúa,Juliana; Machado,Marcos Antonio. |
| In order to obtain a better understanding of what is citrus, 33 cDNA libraries were constructed from different citrus species and genera. Total RNA was extracted from fruits, leaves, flowers, bark, seeds and roots, and subjected or not to different biotic and abiotic stresses (pathogens and drought) and at several developmental stages. To identify putative promoter sequences, as well as molecular markers that could be useful for breeding programs, one shotgun library was prepared from sweet orange (Citrus sinensis var. Olimpia). In addition, EST libraries were also constructed for a citrus pathogen, the oomycete Phythophthora parasitica in either virulent or avirulent form. A total of 286,559 cDNA clones from citrus were sequenced from their 5 end,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESTs; Shotgun; Citrus; Phytopathogen. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500030 |
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| Mehta,Angela; Silva,Marilia Santos; Guidetti-Gonzalez,Simone; Carrer,Helaine; Takita,Marco Aurélio; Martins,Natália F.. |
| Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile). Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Cell signaling; Genomics. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500003 |
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